14.09 (Fri)
Satellite HS Zoologie
15.09 (Sat)
14:00 h Opening of Registration (Kollegiengebäude II; KG II)
18:00 h Welcome address (KG II)
18:30 h H. Ron Kaback (Peter Mitchell Medal Lecture; Audimax)
19:30 h Welcome Reception (Kollegiengebäude I; KG I)
16.09. (Sun)
Chair: P. Gräber
09:00 h P2: ATPase J. Walker
(KG II, Audimax) The ATP synthase: The understood, the uncertain and the unknown
09:30 h P3: ATPase W. Frasch
(KG II, Audimax) Anatomy of FoF1 ATPase-Driven Rotation
10:00 h P4: Membrane Transport P. Nissen
(KG II, Audimax) Structure, function, and regulation of ion pumps
10:30 h coffee break
Chair: U. Brandt
11:00 h P5: Photosynthesis P. Fromme
(KG II, Audimax) Towards the time resolved X-ray structure determination of proteins
11:30 h P6: Photosynthesis W. Rutherford
(KG II, Audimax) Redox tuning in bioenergetics: Compromising efficiency to survive life in O2
12:00 h P7: Photosynthesis J. R. Shen
(KG II, Audimax) High resolution structure of Photosystem II and the mechanism of water-splitting
12:30 h Lunch (Mensa I)
13:30 h Poster viewing
Chair: G. Deckers-Hebestreit Chair: S. Weber Chair: R. Krämer
S1. ATP synthase S2. Photosynthesis S4. Membrane Transporter
(KG II, Audimax) (KG II, SR 2004) (KG II, SR 2006)
14:30 h M. Futai 14:30 h H. Dau 14:30 h J. Herrmann
Rotational Catalysis of E. coli F1 ATPase: rotor/stator interaction Alternating electron and proton transfer in photosynthetic water oxidation Oxidation-driven protein import into the intermembrane space of mitochondria
14:50 h M. Börsch 14:50 h E. Boekma 14:50 h A. Chacinska
Subunit rotation and twisting in FoF1-ATP synthase by single-molecule three-color Förster resonance energy transfer Structure and function of photosynthetic supercomplexes Biogenesis of proteins targeted to the intermembrane space of mitochondria
15:10 h T. Meier 15:10 h R. Blankenship 15:10 h S. Ferguson
Coupling ions to the rotary mechanism and architecture of the FoATP synthase Alternative Complex III from phototrophic bacteria and its electron acceptor auracyanin New perspectives on assembling c-type cytochromes, particularly from sulphate reducing bacteria and mitochondria
15:30 h G. Grüber 15:30 h S. A. Dikanov 15:30 h V. Gold
The crystallographic structure of subunit E from Pyrococcus horikoshii OT3-ATP synthase reveals the flexibility of the peripheral stalk Hydrogen bonding and spin density distribution in the Qb semiquinone of bacterial reaction centers and comparison with the Qa site An electron dense substrate to study mitochondrial import sites in situ
15:45 h P. Pecina 15:45 h M. Brecht 15:45 h T. Pflüger
Role of the mitochondrial ATP synthase central stalk subunits γ and δ in the activity and assembly of the mammalian enzyme Nanoparticles affect the function of photosystem I Regulation of ammonium transport proteins
16:00 h coffee break
Chair: T. Meier Chair: J. Duszynski Chair: M. Rigoulet
S1. ATP synthase S3. Mt Ion pumps S5. UCPs
(KG II, Audimax) (KG II. SR 2004) (KG II. 2006)
16:30 h M. Yoshida 16:30 h S. Hiller 16:30 h R. Porter
Mammalian IF1, an inhibitor of ATP synthase in mitochondria; from single molecule to KO mouse The dynamic compacted state of outer membrane proteins bound to a periplasmic chaperone Role for Ubiquitinylation and the Cytosolic Proteasome in Turnover of Mitochondrial Uncoupling Protein 1 (UCP1)
16:50 h M. F. Giraud 16:50 h A. Szewczyk 16:50 h E. Rial
The yeast ATP-synthase: from its atomic structure to its supramolecular organization in the inner mitochondrial membrane Mitochondrial potassium channels: magnum overview Questions on the activity of UCP2 inhibitors in tumour cells
17:10 h R. Iino 17:10 h D. Siemen 17:10 h W. Jarmuszkiewicz
Rotary catalysis of the stator ring of F1-ATPase Complex Effects of 17β-Estradiol on Mitochondrial Function Purine nucleotides and ubiquinone in mitochondrial energy-dissipation
17:30 h A. K. Rai 17:30 h V. De Pinto 17:30 h C. Hoffmann
ATP synthase from rat liver plasma membrane Beta barrels 9 and 10 are equally important for the gating properties of VDAC1 than the N-terminal domain Evidence for a brown adipocyte specific enhancer in the first intron of the murine Ucp3 gene
17:45 h I. Starke 17:45 h A. Karachitos 17:45 h N. Sommer
The H+-ATPsynthase from Yeast: Construction and Characterisation of an EFGP-MF0F1 Fusion Protein with Cysteine Mutations for FRET Investigations Is huntingtin a modulator of VDAC? Increased pulmonary vascular remodeling in UCP2 deficiency
20:00 h Rock concert Bill Rutherford (@Peterhofkeller)
17.09. (Mon)
Chair: T. Friedrich
09:00 h P8: Complex I U. Brandt
(KG II, Audimax) Mitochondrial Complex I: Insights from Structural and Functional Studies
09:30 h P9: Complex I L. Sazanov
(KG II, Audimax) Structure and mechanism of complex I
10:00 h P10: Mt. disease D. Wallace
(KG II, Audimax) A Mitochondrial Etiology of Complex Diseases: Verification through Mouse Models
10:30 h coffee break
Chair: M. Borner
11:00 h P11: Mt. disease R. Youle
(KG II, Audimax) Damage Control - How the Pink1/Parkin pathway can regulate removal of impaired mitochondria by autophagy
11:30 h P12: Mt. Supercomplexes W. Kühlbrandt
(KG II, Audimax) Structure of the ATP synthase and respiratory chain complexes in the inner mitochondrial membrane
12:00 h P13: Electron transfer L. R. Dutton
(KG II, Audimax) Electron tunneling in biological energetics
12:30 h Lunch (Mensa I)
13:30 h Poster viewing
Chair: P. Rich Chair: C. Meisinger Chair: L. Kussmaul
S6. Complex I S7. Biogenesis S9. Mitochondria and neuronal function
(KG II, Audimax) (KG II, SR 2004) (KG II, SR 2006)
14:30 h J. Hirst 14:30 h L. Nijtmans 14:30 h K. Winklhofer
New information about the molecular mechanism of mitochondrial complex I The making of mitochondrial Complex I: a key in regulating metabolism Parkin: A stress-protective E3 ubiquitin ligase maintaining mitochondrial integrity
14:50 h M. Wikström 14:50 h E. Glaser 14:50 h G. Marsicano
On the mechanistic stoichiometry of proton translocation by respiratory complex I The mitochondrial Presequence Protease, PreP, and Alzheimer’s disease Cannabinoid type-1 receptors in brain mitochondria
15:10 h T. Ohnishi 15:10 h H. G. Koch 15:10 h A. Pujol
EPR detection of two protein-associated ubiquinone components (SQNf and SQNs( in the membrane in situ and in proteoliposomes of isolated bovine heart complex I Biogenesis of cytochrome cbb3 Oxidase: analysis of the copper delivery pathway Mitochondria failure underlying axonal degeneration in adrenoleukodystrophy, a peroxisomal disease.
15:30 h S. de Vries 15:30 h R. Lill 15:30 h L. D. Ossellame
Initial electron transfer steps in complex I Energetic basis of the biogenesis of iron-sulfur proteins Accumulation of damaged mitochondria in neuropathic Gaucher disease, the most prevalent risk factor for Parkinson’s disease, is due to defective cellular degredation machinery
15:45 h M. Babot 15:45 h T. Becker 15:45 h C. Pellegrini
Mapping of A/D conformational changes of mitochondrial complex I Biogenesis of alpha-helial outer membrane proteins Melanocytes - A novel tool to study mitochondrial dysfunction in Duchenne muscular dystrophy
16:00 h coffee break
Chair: J. Hirst Chair: N. Pfanner Chair: O. Einsle
S6. Complex I S8. Mitochondrial dynamics S10. Transporters
(KG II, Audimax) (KG II. SR 2004) (KG II. 2006)
16:30 h M. Pereira 16:30 h E. Hollville 16:30 h M. Quick
New perspective on the relations between complex I, [NiFe] hydrogenases and Na+/H+ antiporters and respective evolutionary, functional and mechanistic implications Bcl-2 family proteins and mitochondrial quality control Transport in slow motion
16:50 h J. Steuber 16:50 h M. Ryan 16:50 h S-J. Woo
The Na+-translocating NADH: quinone oxidoreductase (Na+-NQR): Localization of a quinone binding site and structure at 16 Å resolution Characterisation of MiD49 and MiD51 in mitochondrial division X-ray structure of a bacterial ABC-type heme transporter
17:10 h B. Barquera 17:10 h B. Westermann 17:10 h C. Ziegler
Insights into the mechanism of electron transfer and sodium translocation of the Na+-pumping NADH:quinone oxidoreductase Mitochondrial dynamics in yeast Na+-coupling in the osmoregulated betaine transporter BetP
17:30 h I. Wittig 17:30 h R. Krüger 17:30 h F. Marinelli
Identification of new assembly factors of complex I by complexome profiling Impaired mitochondrial dynamics in neurodegeneration in Parkinson’s disease Insights into the mechanism of the Na+/Ca2+ exchanger from atomistic molecular dynamics simulations
17:50 h M. Murai 17:50 h S. Melser 17:45 h R. A. Oot
Characterization of the inhibitor-binding site in mitochondrial NADH-Ubiquinone oxidoreductase (complex I) Mitophagy mediated by Rheb and the maintenance of mitochondrial activity Crystal structure of the heterotrimeric EGChead complex from yeast vacuolar ATPase
18:05 h G. Manjeri
Tissue specific metabolic consequences of isolated mitochondrial complex I deficiency in mice
20:00 h Conference diner in honor of Ron Kaback
18.09. (Tue)
Chair: C. Hunte
09:00 h P14: Transhydrogenase B. Jackson
(KG II, Audimax) The binding-change mechanism of proton-translocating transhydrogenase
09:30 h P15: Bc1 complex A. Osyczka
(KG II, Audimax) Electron and proton transfers in the catalytic mechanism of cytochrome bc1
10:00 h P16: Bc1 complex M. Teixera
(KG II, Audimax) The alternative complex III: Properties and possible mechanisms for electron transfer and energy conservation
10:30 h coffee break
Chair: L. Wojtczak
11:00 h P17: Proton circuits D. Nicholls
(KG II, Audimax) Mitochondria and the control of insulin secretion
11:30 h P18: Signalling P. Bernardi
(KG II, Audimax) Progress on the mitochondrial permeability transition pore and its role in pathophysiology
12:00 h P19: Signalling E. R. Bamberg
(KG II, Audimax) Microbial Rhodopsins: from bioenergetic aspects to optogenetics
12:30 h Lunch (Mensa I)
Chair: C. Borner Chair: J. P. Mazat Chair: A. Bechthold
S11. Signalling S12. bc1 and quinones S9. ROS and ageing
(KG II, Audimax) (KG II, SR 2004) (KG II, SR 2006)
13:30 h C. Mammucari 13:30 h M. Ott 13:30 h M. Brand
Mitochondrial Ca2+ signaling in skeletal muscle: molecules and roles Early steps in the biogenesis of cytochrome b in yeast mitochondria Mitochondrial superoxide production under native conditions
13:50 h J. Duszynski 13:50 h F. Daldal 13:50 h L. Tretter
Effect of mtDNA point mutations on cellular bioenergetics Inter-monomer Electron Transfer Between the b Hemes of Cytochrome bc1 Reactive oxygen species generation by mitochondrial dehydrogenases under pathological conditions
14:10 h F. Gellerich 14:10 h F. Baymann 14:10 h A. Vinogradov
The mitochondrial gas pedal: Cytosolic calcium regulates energisation of neuronal mitochondria via pyruvate formation by the malate aspartate shuttle Evolution of Rieske/cytochrome b complexes, the enzyme that witnessed the history of life since the Last Universal Common Ancestor Mitochondrial production of reactive oxygen species: progress in understanding
14:30 h C. Meisinger 14:30 h D. Picot 14:30 h J. Nedergaard
Signalling Pathways Regulating Mitochondrial Protein Import The mysterious cofactors of the cytochrome b6f complex: contribution of C. rheinhardtii mutants to structure/function relationships Mild uncoupling to counteract ROS production: physiological relevant or not
14:50 h U. Schlattner 14:45 h L. B. Valdez 14:50 h B. Daum
Mitochondrial Nm23-H4 can switch between phosphotransfer and lipid transfer activities Inhibition of mitochondrial complex III by nitric oxide Macromolecular reorganization of mitochondria during ageing
COST meeting
Chair: F. MacMillan
Translocation of protons across biomembranes
15:00 h EMBO reports workshop 15:00 h H. Sychrova
Point mutations change the substrate specificity of yeast plasma-membrane Na+/H+ antiporters
15:15 h A. Y. Mulkidjanian
On the mechanism of proton leakage through phospholipid bilayer
15:30 h D. V. Dibrova
Co-evolution of fatty acid biosynthesis and membrane proton-translocating complexes
15:45 h J. ter Beek
Defining and manipulating proton transfer pathways in nitric oxide reductase
16:00 h Poster viewing 16:00 h C. von Ballmoos
Variable proton-pumping stoichiometries in cytochrome c oxidases
16:15 h B. Meunier
Generation of mutants of yeast cytochrome oxidase
16:30 h A. Marechal
Possible interactions between subunit 5A/B and the H channel of subunit I of yeast cytochrome oxidase
16:45 h General discussion
17:00 h free time (City tours)
20:00 h Banquet@Schloss Reinach
19.09. (Wed)
Chair: M. Wikström
09:00 h P20: Oxidase H. Michel
(KG II, Audimax) Cytochrome c oxidases of the A and C families: structures and mechanisms
09:30 h P21: Oxidase J. Collman
(KG II, Audimax) Reversibly modulating Cytochrome c Oxidase inhibits blood clotting: studies based on functional electrochemical models and respiring cells
10:00 h P22: Oxidase S. Fergusson-Miller
(KG II, Audimax) Role of conformational change and lipidic ligands in the function and regulation of cytochrome c oxidase
10:30 h coffee break
Chair: P. Bernardi
11:00 h P23: Physiology L. Scorrano
(KG II, Audimax) Keeping mitochondria in shape: a matter of life and death
11:30 h P24: Physiology W. Kunz
(KG II, Audimax) ROS production and turnover of brain mitochondria - implications for neurological diseases
12:00 h P25: Mt-DNA J-P. di Rago
(KG II, Audimax) Experimental relocation of the mitochondrial ATP9 gene to the nucleus reveals forces underlying mitochondrial genome evolution
12:30 h EBEC 2014 M. Pereira
12:40 h Lunch (Mensa I) (Delegates meeting@Oberkirch)
13:30 h Poster viewing
Chair: S. de Vries Chair: A. Szewczyk Chair: S. Andrade
S14. Terminal oxidases* S15. mt Physiology S16. Protein/lipids interactions
(KG II, Audimax) (KG II, SR 2004) (KG II, SR 2006)
14:30 h R. Gennis 14:30 h W. Koopman 14:30 h A. Magalon
The Diversity of the Heme-copper Superfamily: O2 and NO reductases ROS as signalling molecules in mitochondrial and cellular (dys)function Membrane-bound respiratory nitrate reductase functioning: it takes two to tango
14:50 h T. Soulimane 14:50 h T. Frey 14:50 h P. Hellwig
Structure of caa3-cytochrome c oxidase - A nature-made enzyme-substrate complex Insights into mitochondrial structure and function from correlated four-dimensional light and electron microscopy Lipid protein interaction in enzymes from the respiratory chain: an electrochemical and FTIR spectroscopic study on the role of integral lipids
15:10 h Y. Shiro 15:10 h R. Rossignol 15:10 h J. Borrell Hernandez
Structural basis for NO reduction by bacterial nitric oxide reductases Mitoplasticity: adaptation of mitochondrial form and function to the redox status of the cell Influence of acyl chain composition on phosphatidylethanolamine-lactose permease interaction: a combined AFM and FRET study
15:30 h S. Nakashima 15:30 h T. Wenz 15:30 h E. Svahn
Nanosecond time-resolved infrared basis for a bulge of the transmembrane helix between hemes a and a2 to facilitate highly efficient proton pumping by bovine heart cytochrome c oxidase Sirt3 affects the function and stability of OXPHOS complexes in response to metabolic challenges The membrane modulates internal proton transfer in cytochrome c oxidase
15:45 h D. A. Bloch 15:45 h M. Y. Vyssokikh 15:45 h M. Abe
Cytochrome bd-II from aerobic respiratory chain of Escherichia coli is a proton-motive force generator Isoform porin 2 is involved in tRNALys transport from cytosol to mitochondria in yeast Synthesis of cardiolipin analogs and characterization of their effects on induction of the peroxidase activity of cytochrome c
16:00 h L. Grossman 16:00 h A. Rasola 16:00 h K. Gieczewska
Gene regulation of cytochrome c oxidase subunit 4 isoform 2: A tale of three factors The mitochondrial chaperone TRAP1 promotes neoplastic growth by inhibiting succinate dehydrogenase Does lipid saturation change affect the aggregation of proteoliposomes in semi-lemellar system?
16:15 h coffee break
Chair: P. Brzezinski Chair: R. Blankenship Chair: A. Mulkidjanian & C. Hägerhall
S14. Terminal oxidases* S2. Photosynthesis S21. Phylogenomics of energy-converting enzymes
(KG II, Audimax) (KG II. SR 2004) (KG II. 2006)
16:45 h P. Ädelroth 16:45 h J. Messinger 16:45 h C. Hägerhall
Proton transfer pathways in the heme-copper oxidase superfamily Photosynthetic water-oxidation: approaching a molecular understanding Phylogenomics of energy-converting enzymes - sorting out the pieces of a molecular meccano
17:05 h P. Rich 17:05 h O. Lenz 17:05 h M. Galperin
Yeast cytochrome c oxidase: a link between structures and mechanisms of bacterial and mammalian forms Oxygen-tolerant hydrogenase - the centerpiece of the microbial hydrogen combustion engine Phylogenomic approaches to proton- and sodium-transporting ATPases
17:25 h A. Moore 17:25 h H.P. Braun 17:25 h D. Dibrova
Crystal structure of the alternative oxidases: New insights into the catalytic cycle The mitochondrial complex I of plants: Function in respiration and photosynthesis Evolution of lipid biosynthesis may have driven the evolution of membrane bioenergetics
17:45 h S. K. Choi 17:45 h M. Guiral 17:45 h H. H. Luoto
Investigations of the proton pump and exit pathway in cytochrome ba3 from Thermus thermophilus The energy sulfur metabolism of the hyperthermophilic bacterium Aquifex aeolicus The evolutionary history of membrane-integral pyrophosphatases supports Na+ as the ancestral coupling ion in membrane bioenergetics
18:00 h Y. Gao 18:05 h A. Dlaskova 18:00 h B. C. Marreiros
Heme-copper terminal oxidases using both cytochrome c and ubiquinol as electron donors Visualization of mt nucleoids by superresolution microscopy techniques Taxonomic profile of complex I, group 3 [NiFe] hydrogenases and related complexes
18:15 h F. Melin 18:20 h A. Indrieri 18:15 h M. F. Yanyushin
Electrochemical analysis of cytochrome ba3 from T. thermophilus immobilized on gold nanoparticles Severly impaired respiratory chain causes multisystem apoptosis-driven developmental defects, a new mitochondrial phenotype in vertebrates Two types of quinol:periplasmatic e-transporter oxidoreductases. Comparison of their operon variations and distribution through bacterial phyla
18:30 h J. Hemp
The heme-copper oxidoreductase superfamily: Diversity, evolution and ecology
* Supported by the COST Action CM0902 “Molecular machineries for ion translocation across biomembranes”
20.09. (Thu)
Chair: B. Ludwig
09:00 h P26: Mt. Biology R. Balaban
(KG II, Audimax) Dynamic Imaging of Mitochondrial Function in vivo
09:30 h P27: Mt. Biology W. Neupert
(KG II, Audimax) Biogenesis and the molecular architecture of mitochondria
10:00 h P28: General bioenergetics W. Junge
(KG II, Audimax) Molecular Bioenergetics and Beyond
Chair: C. Mullinaux Chair: R. Balaban Chair: G. Cecchini
S18. Supercomplexes/Proteomics S17. System bioenergetics S20. ET and Proton translocation
(KG II, Audimax) (KG II, SR 2004) (KG II, SR 2006)
10:35 h M. L. Genova 10:35 h M. Rigoulet 10:35 h A. Stuchebrukhov
Advantages of the supramolecular organisation of the respiratory chain: kinetic improvement and limited production of reactive oxygen species Dehydrogenase kinetic parameters determine the electron competition mechanisms in respiratory chain Electron tunneling and proton translocation in respiratory complex I
10:55 h M. Rugolo 10:55 h J. Keltjens 10:55 h T. Koslowski
Alterations in the supramolecular interactions of respiratory chain complexes and enhanced superoxide production by the cytochrome b Y278C mutation which causes a multisystem disorder To make a living from anaerobic ammonium oxidation (anammox) Recent progress in biological charge transfer theory and simulation
11:15 h K. Davies 11:15 h M. Nooteboom 11:15 h M. Malferrari
Assessing the structure of supercomplexes 'in situ' Combining quantitative live-cell microscopy with mathematical modeling to understand and combat mitochondrial disease in skeletal muscle Exploring the coupling between electron transfer and protein dynamics in photosynthetic reaction centers embedded into dehydrated amorphous matrices
11:30 h coffee break
12:00 h C. Ugalde 12:00 h J. Faraldo-Gomez 12:00 h J.P. Mazat
Mitochondrial complex I plays as essential role in human respirasome assembly Structure, Specificity and Membrane Organization of ATP synthases Virtual Mitochondrion: A multi-level modeling of mitochondrial bioenergetics
12:20 h L. N. Liu 12:20 h R. Springett 12:20 h M. Ullmann
Control of electron transport routes through redox-regulated redistribution of respiratory complexes The relative contribution of membrane potential and pH gradient regulates mitochondrial oxygen consumption at constant proton motive force pKa Values and Redox Potentials of Proteins - What do they mean
12:35 h D. B. Papkovsky 12:35 h P. Pohl
Control of oxygenation and bioenergetic assessment of respiring objects with intracellular oxygen-sensing probes Interfacial water provides the pathway for proton transport along membranes